分类： 生物学 >> 生物物理学 >> 生物物理、生物化学与分子生物学 提交时间： 2016-05-12
摘要： With the rapid development of high-throughput sequencing technologies, the emerging of methylated RNA immunoprecipitation sequencing (MeRIP-seq) technology makes it possible to detect RNA epigenetic modifications in a large scale, which allows transcriptome-wide profiling of RNA methylation. Mining the patterns of global mRNA methylation from these MeRIP-seq data can help reveal the potential functional roles of these mRNA methylations in regulating gene expression, splicing, RNA editing and RNA stability, effectively guiding the therapeutic intervention of cancer. Here, the principle of MeRIP-seq sequencing was first introduced. Then, the recent progress of the processing and analysis of MeRIP-seq data were comprehensively discussed. In the end, the computational problems and challenges faced in the process of MeRIP-seq data processing were also summarized.
分类： 生物学 >> 生物物理学 >> 生物物理、生物化学与分子生物学 提交时间： 2016-05-11
摘要： Caste determination in the honeybee has been demonstrated to be decided by the royalactin in the royal jelly. And the differentially expressed coding genes in caste determination of the worker and queen larvae have also been well studied. Our experiments identified that lncRNAs are also differentially expressed in the caste determination of the larvae, which indicates that lncRNAs are involved in the caste determination. Detailed analysis showed that in the early development of the larvae, lncRNAs play a regulatory role in multicellular organismal development, nervous system development and regulation of transcription by affecting the expression and function of the nearby coding genes.
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